Formaldehyde Fixation of Extracellular Matrix Protein Layers for Enhanced Primary Cell Growth

Formaldehyde Fixation of Extracellular Matrix Protein Layers for Enhanced Primary Cell Growth

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Coating tissue culture vessels with the components of the extracellular matrix such as fibronectin and collagens provides a more natural environment for primary cells in vitro and stimulates their proliferation.However, the effects of such protein layers coq-clear 100 ubiquinol are usually rather modest, which might be explained by the read more loss immobilized proteins due to their weak non-covalent association with the tissue culture plastic.Here we describe a simple protocol for a controlled fixation of fibronectin, vitronectin and collagen IV layers by formaldehyde, which substantially enhances the stimulation of primary cell proliferation by these extracellular proteins.